High Pressure Homogenizer for Biotechnology

In the field of biopharmaceuticals, the efficient recovery of intracellular products such as recombinant proteins, enzymes, plasmid DNA, and antibodies is the core of downstream processes. High-pressure homogenization technology is the standard for large-scale, industrial-level cell disruption applications. This homogenizer uses intense mechanical force to efficiently break open the cell walls of microorganisms (e.g., E. coli and yeast) and release the target components while preserving their biological activity. ATS provides the biopharmaceutical industry with high-performance and reliable high-pressure cell disruption solutions, ensuring seamless scale-up from laboratory to industrial-scale cGMP production.

Advantages of ATS High-Pressure Homogenizer

Efficient High-Density Cell Disruption
Capable of processing highly concentrated (high-density fermentation) bacterial and yeast suspensions at extremely high pressures of 2000 bar or higher, resulting in effective and efficient cell disruption.
Biological Activity Preservation
ATS high-pressure homogenizer is equipped with a heat exchange system for precise temperature control. It is essential to maintain a stable temperature during processing to prevent target proteins from denaturing or degrading due to temperature increases during disruption, thereby maximizing sample activity and yield.
Excellent Repeatability and Scalability
Homogenization results (disruption rate, energy consumption, and temperature rise) are highly consistent across batches. With the expertise on fluid dynamics models, small-scale process parameters can be predictably and linearly scaled up to industrial scale, ensuring scalability while significantly reducing process transfer risks.
Full GMP Compliant
The high-pressure homogenizer is specifically designed to meet pharmaceutical product quality guidelines, including sterile production, traceability, and validation under cGMP.

Recommended Products for Cell Disruption

High Pressure Homogenizers

High disruption rate, with a single process achieving a disruption rate of over 99%

Full temperature control to preserve biological activity

Variable flow control system for adjustable flow rates according to requirements

GMP-compliant design and CE-certified

Customizable configurations for PLC/explosion-proof/sterile requirements

Four-layer overpressure protection ensures operational safety

Scalability from laboratory to industrial, with a maximum flow rate of 4000 L/H

Customer Cases

Experimental Cases

Lactobacillus cell disruption

Equipment used
Laboratory high-pressure homogenizer
Experimental results
After the disruption, the Lactobacillus sample became clearer. Microscope observation of the disruption result showed that the Lactobacillus cells were mostly broken apart.

Microscopic view

ATS High Pressure Homogenizer for Rhodotorula and Phaffia Yeasts Disruption

Experimental background
Rhodotorula and Phaffia yeasts are the earliest strains used in the production of astaxanthin and are the most suitable microorganisms for producing astaxanthin besides Haematococcus pluvialis. The produced astaxanthin has a clockwise (R enantiomer) configuration, possessing certain antioxidant activity.
Equipment
Pilot-scale high pressure homogenizer AH-PILOT series
Experimental results
Visual observation
The disrupted Rhodotorula and Phaffia yeast became more viscous and turned orange, indicating that the astaxanthin was released.

Microscopic view

Chlorella cell disruption

Background
Chlorella is an alkaline food rich in various balanced nutrients. However, these substances are encapsulated by the cell wall and cannot be directly utilized. The cell wall must be broken to release these nutrients for extraction and use.
Experimental process
Using ATS high-pressure homogenizer to disrupt the cell wall under high pressure and low temperature.
Experimental objective
Achieve a disruption rate of ＞90%
Experimental results
Visual observation
The Chlorella cell remained stable without any stratification after disruption.